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General Information
Symbol
M{UAS-RpL14.dsRNA.RpL14r}
FlyBase ID
FBtp0093908
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Dmau\mariner
Construct components
Component allele RpL14dsRNA.UAS.RpL14-r
Product class / Tool use(s)
Description

This construct contains two elements. First, UAS regulates expression of a dsRNA corresponding to a 72bp non-contiguous region on exon 2 of RpL14, that targets endogenous RpL14 transcripts for RNAi-mediated knock-down. Second, a complete RpL14 genomic region (called "RpL14[r]") containing 14 synonymous mutations in exon 2 which confer insensitivity to the RNAi induced by the first element. The genomic region includes fragments of flanking genes to help ensure that all regulatory regions are included, and two intronic snoRNAs.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
Component Alleles
Reference(s)
Molecular data

This construct contains two elements. First, UAS regulates expression of a dsRNA corresponding to a 72bp non-contiguous region on exon 2 of RpL14, that targets endogenous RpL14 transcripts for RNAi-mediated knock-down. Second, a complete RpL14 genomic region (called "RpL14[r]") containing 14 synonymous mutations in exon 2 which confer insensitivity to the RNAi induced by the first element. The genomic region includes fragments of flanking genes to help ensure that all regulatory regions are included, and two intronic snoRNAs.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
M{UAS-RpL14.dsRNA.RpL14r}
{UAS-RpL14.dsRNA, RpL14[r]}
Secondary FlyBase IDs
    References (2)