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General Information
Symbol
P{UASp-NLS-V5-TEV.S219V}
FlyBase ID
FBtp0096062
Feature type
transgenic_transposon
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Description

UASp regulatory sequences drive expression of TEV\NIa protease, tagged at the N-terminal end with a nuclear localisation signal, and a Tag:V5 tag. The TEV\NIa protein contains the amino acid replacement S219V, which inhibits self-cleavage and results in approximately two-fold higher activity levels (see PMID:11809930).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
molecular
tag
molecular
tag
Component Alleles
Reference(s)
Molecular data

UASp regulatory sequences drive expression of TEV\NIa protease, tagged at the N-terminal end with a nuclear localisation signal, and a Tag:V5 tag. The TEV\NIa protein contains the amino acid replacement S219V, which inhibits self-cleavage and results in approximately two-fold higher activity levels (see PMID:11809930).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UASp-NLS-V5-TEV.S219V}
Secondary FlyBase IDs
    References (2)