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General Information
Symbol
P{lox(Trojan-GAL4)x3}
FlyBase ID
FBtp0099339
Feature type
transgenic_transposon
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Description

P{lox(Trojan-GAL4)x3} is a transgenic donor construct containing three tandem 'Trojan GAL4' cassettes, one in each intron phase. The Trojan GAL4 sequence in each case consists of a splice acceptor site followed by the T2A peptide, the GAL4 coding sequence and an Hsp70 transcription termination signal. Each cassette is flanked by attB sites nested within a pair of uniquely compatible lox sites (lox2272 for the phase 0 cassette, loxN for the phase 1 cassette, and loxP for the phase 2 cassette). Expression of P1cre recombinase will excise and circularise all three cassettes, which can then be integrated into the attP sites of a Mi{MIC} insertion using phiC31:int integrase. Integration of the cassette with the appropriate phase into a coding intron of a native Drosophila gene of interest will result in the cassette behaving as a 'Trojan' exon: the splice acceptor site ensures that the T2A-GAL4 open reading frame is incorporated into the mRNA of the native Drosophila gene, while the T2A sequence truncates the native gene product and promotes the separate translation of the GAL4 open reading frame. Thus GAL4 should be expressed under the control of the regulatory sequences of the native Drosophila gene of interest in the resulting Trojan-MiMIC GAL4 driver line.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
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Total Size
Left end
Right end
 
Segments
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Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (3)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lox(Trojan-GAL4)x3}
triplet donor transgene
Secondary FlyBase IDs
    References (3)