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General Information
Symbol
Mi{Trojan-GAL4.0}
FlyBase ID
FBtp0099343
Feature type
transgenic_transposon
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
GAL4 gene trap
Description
CV term
Qualifiers and info
Reference
transposon
transposon_modified_in_vivo
Construct components
Product class / Tool use(s)
Description

Mi{Trojan-GAL4.0} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with an intron phase 0 'Trojan GAL4' cassette. The donor for the Trojan GAL4 cassette can be any one of the following: a microinjected plasmid (pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70) or an insertion of a transgenic construct (P{lox(Trojan-GAL4)x3} or P{loxP(Trojan-GAL4.0)}). The Trojan GAL4 cassette consists of a splice acceptor site followed by the T2A peptide, the GAL4 coding sequence and an Hsp70 transcription termination signal. Integration of the cassette into a Mi{MIC} insertion in a coding intron (with the same phase) of a native Drosophila gene of interest will result in the cassette behaving as a 'Trojan' exon: the splice acceptor site ensures that the T2A-GAL4 open reading frame is incorporated into the mRNA of the native Drosophila gene, while the T2A sequence truncates the native gene product and promotes the separate translation of the GAL4 open reading frame. Thus GAL4 should be expressed under the control of the regulatory sequences of the native Drosophila gene of interest in the resulting Trojan-MiMIC GAL4 driver line.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

One of 3 essentially identical transgenic constructs (Mi{Trojan-GAL4.0}, Mi{Trojan-GAL4.1} and Mi{Trojan-GAL4.2}) that vary only in the phase of the Trojan GAL4 cassette.

Stocks (262)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Mi{Trojan-GAL4.0}
Secondary FlyBase IDs
    References (9)