FlyBase Recombinant Construct Report: P{10xUAS-IVS-myr::smGdP-OLLAS}

Ten copies of the UAS regulatory sequence drive expression of a non-fluorescent 'spaghetti monster GFP' ('smGdP') marker which consists of 10 copies of the Tag:OLLAS epitope inserted in groups into the backbone of the superfolder green fluorescent protein, which has been rendered non-fluorescent by mutation. A N-terminal myristoylation signal (Tag:Myr(Src64B)) is also present, to target the protein to the plasma membrane, and the marker has been optimised for Drosophila codon usage.

(Nern et al., 2015)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^{10xUAS.smGdP.Tag:Myr(Src64B),Tag:OLLAS}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

P{10xUAS-IVS-myr::smGdP-OLLAS}

(FlyBase, 1992-)

pJFRC209-10xUAS-IVS-myr::smGFP-OLLAS

(Nern et al., 2015)

Secondary FlyBase IDs

References (2)

Report Sections

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