FlyBase Recombinant Construct Report: PBac{UAS-BiP-sfGFP-HDEL}

UASt regulatory sequences drive expression of a fluorescent endoplasmic reticulum (ER) lumen marker. This consists of the Tag:SS(BiP) signal sequence (which traffics the protein to the ER), superfolder GFP (a form of GFP that can fold properly in oxidising environments such as the ER lumen), and a C-terminal ER retention signal (Tag:ER(HDEL)).

(Summerville et al., 2016)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^sfGFP.ER.UAS

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Scer\UAS regulatory sequences drive expression of a fluorescent endoplasmic reticulum (ER) lumen marker. This consists of the T:SS-BiP signal sequence (which traffics the protein to the ER), superfolder GFP (a form of GFP that can fold properly in oxidising environments such as the ER lumen), and a C-terminal ER retention signal (T:ER-HDEL).

(Summerville et al., 2016)

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

64748

w¹¹¹⁸; PBac{UAS-BiP-sfGFP-HDEL}VK00037

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PBac{UAS-BiP-sfGFP-HDEL}

(FlyBase, 1996-)

UAS-BiP-sfGFP-HDEL

(Summerville et al., 2016)

UAS-sfGFP-HDEL

(Sung and Lloyd, 2024)

Secondary FlyBase IDs

References (3)

Report Sections

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