FB2021_01, released Feb 18, 2021

Recombinant Construct: P{UAS-Nmnat.4-7.AltReport}

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General Information
Symbol
P{UAS-Nmnat.4-7.AltReport}
FlyBase ID
FBtp0112830
Feature type
transgenic_transposable_element
Component Allele(s)
Nmnat4-7.AltReport.UAS.DsRed(Unk),AcGFP1
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
(Ruan et al., 2015)
Construct components
Component allele Nmnat4-7.AltReport.UAS.DsRed(Unk),AcGFP1
Product class / Tool use(s)
Regulatory region(s)
UAS
Tagged with
AcGFP1
DsRed(Unk)
Description

UAS regulatory sequences drive expression of exons 4-7 of Nmnat with a DsRed(Unk) tag inserted in exon 5 and an AcGFP (AcGFP1) tag inserted in exon 6. This construct will produce two alternative transcripts: one containing the DsRed(Unk) sequence (proxy for splicing to RB) and one containing the AcGFP1 sequence (proxy for splicing to RA).

(Ruan et al., 2015)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
(Ruan et al., 2015)
molecular
tag
(Ruan et al., 2015)
molecular
tag
(Ruan et al., 2015)
Component Alleles
Allele
Nmnat4-7.AltReport.UAS.DsRed(Unk),AcGFP1
Reference(s)
(Ruan et al., 2015)
Molecular data

UAS regulatory sequences drive expression of exons 4-7 of Nmnat with a DsRed(Unk) tag inserted in exon 5 and an AcGFP (AcGFP1) tag inserted in exon 6. This construct will produce two alternative transcripts: one containing the DsRed(Unk) sequence (proxy for splicing to RB) and one containing the AcGFP1 sequence (proxy for splicing to RA).

(Ruan et al., 2015)
Phenotype Data
Phenotypic ClassPhenotype Manifest InDetailed Description
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Nmnat.4-7.AltReport}
(FlyBase, 1992-)
UAS-DmNMNAT4-7 AltReport
(Ruan et al., 2015)
Secondary FlyBase IDs
    References (2)