FlyBase Recombinant Construct Report: P{R35D04-p65.AD}

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Recombinant Construct: P{R35D04-p65.AD}

General Information

Symbol

P{R35D04-p65.AD}

FlyBase ID

FBtp0117187

Feature type

transgenic_transposable_element

Component Allele(s)

Hsap\RELA^AD.R35D04

Also Known As

R35D04-p65.AD, R35D04-p65ADZp

Size

Expression Data

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component allele Hsap\RELA^AD.R35D04

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

split driver - transcription activation fragment

Description

The R35D04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Wu et al., 2016)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Hsap\RELA^AD.R35D04

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

lobula columnar neuron LC10a

Comment: when combined with P{R22D06-GAL4.DBD} (combination referred to as 'OL0019B')

when combined with P{VT043656-GAL4.DBD} (combination referred to as 'SS2')

when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

when combined with P{R80E07-GAL4.DBD} (combination referred to as 'OL0020B')

when combined with P{R82D11-GAL4.DBD} (combination referred to as 'OL0021B')

when combined with P{R91B01-GAL4.DBD} (combination referred to as 'OL0022B')

when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

(Ribeiro et al., 2018, Wu et al., 2016)

lobula columnar neuron LC10b | subset

Comment: when combined with P{VT043656-GAL4.DBD} (combination referred to as 'SS2')

when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

(Ribeiro et al., 2018, Wu et al., 2016)

lobula columnar neuron LC10a | subset

Comment: when combined with P{R22A07-GAL4.DBD} (combination referred to as 'SS00940')

(Wu et al., 2016)

lobula columnar neuron LC10b

Comment: when combined with P{R22A07-GAL4.DBD} (combination referred to as 'SS00940')

when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

when combined with P{R82D11-GAL4.DBD} (combination referred to as 'OL0021B')

when combined with P{VT040747-GAL4.DBD} (combination referred to as 'SS02663')

when combined with P{VT043656-GAL4.DBD} (combination referred to as 'SS02664')

when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

(Wu et al., 2016)

lobula columnar neuron LC10c

Comment: when combined with P{R22A07-GAL4.DBD} (combination referred to as 'SS00940')

when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

when combined with P{R82D11-GAL4.DBD} (combination referred to as 'OL0021B')

when combined with P{VT040747-GAL4.DBD} (combination referred to as 'SS02663')

when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

(Wu et al., 2016)

lobula columnar neuron LC12

Comment: when combined with P{R55F01-GAL4.DBD} (combination referred to as 'OL0008B')

when combined with P{R65B05-GAL4.DBD} (combination referred to as 'OL0007B')

(Klapoetke et al., 2022, Özel et al., 2021, von Reyn et al., 2017, Wu et al., 2016)

adult ventral nerve cord | restricted

Comment: when combined with P{R55F01-GAL4.DBD} (combination referred to as 'OL0008B')

when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

(Wu et al., 2016)

lobula columnar neuron LC10d

Comment: when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

when combined with P{R80E07-GAL4.DBD} (combination referred to as 'OL0020B')

when combined with P{R91B01-GAL4.DBD} (combination referred to as 'OL0022B')

when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

(Wu et al., 2016)

lobula columnar neuron LC10c | subset

Comment: when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

(Wu et al., 2016)

medulla dorsal rim area-posterior lateral protocerebrum neuron

Comment: when combined with P{R65B05-GAL4.DBD} (combination referred to as 'OL0007B')

(Kind et al., 2021)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Images

Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (14)

Stocks containing insertion(s) of this recombinant construct

601241

w¹¹¹⁸; P{R35D04-p65.AD}JK73A/TM3, Sb¹

68336

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{R22D06-GAL4.DBD}attP2

68337

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{R80E07-GAL4.DBD}attP2

68352

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{R65B05-GAL4.DBD}attP2

68353

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{R55F01-GAL4.DBD}attP2

68355

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{R82D11-GAL4.DBD}attP2

68361

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{R91B01-GAL4.DBD}attP2

68378

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{R71E06-GAL4.DBD}attP2

68381

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{R22A07-GAL4.DBD}attP2

68339

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{VT043656-GAL4.DBD}attP2

68343

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{VT040747-GAL4.DBD}attP2

68344

w¹¹¹⁸; P{R35D04-p65.AD}attP40; P{VT058944-GAL4.DBD}attP2

More stocks available...

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P{R35D04-p65.AD}

(FlyBase, 1992-)

R35D04-p65.AD

(Praschberger et al., 2023, Nojima et al., 2021, Bidaye et al., 2020)

R35D04-p65ADZp

(Klapoetke et al., 2022, Kind et al., 2021, Davis et al., 2020)

R35D04.p65ADZp

(Ribeiro et al., 2018)

R35D04AD

(Kim et al., 2023)

R35D04_p65ADZp

(von Reyn et al., 2017)

Secondary FlyBase IDs

References (18)

Report Sections