FB2025_01 , released February 20, 2025
Recombinant Construct: M{slam.PP7}
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General Information
Symbol
M{slam.PP7}
FlyBase ID
FBtp0126848
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele slamPP7
Transgenic product class
Component
Use(s)
Regulatory region(s)
Encoded product / tool
Description

Genomic fragment containing slam expressed under its own regulatory sequences. 12 copies of the Tag:PP7 stem loop sequence have been inserted towards the 3' end of the coding sequence, at position 3460. Six copies of the Tag:MS2 stem loop have been inserted into the 3'UTR. The Tag:PP7 stem loop RNA sequence can be specifically bound by the Tag:PCP protein. The Tag:PP7 sequence is also translatable. These properties allows this construct to be used as a reporter of slam translation in vivo: prior to translation, the mRNA can be bound by a reporter protein with a functional readout (such as fluorescence, bioluminescence or enzymatic activity) containing Tag:PCP, but during the first round of translation, this reporter protein is knocked off the mRNA by the ribosome. The resulting change in the functional readout of the reporter can be used to study the dynamics of translation.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{slam.PP7}
Secondary FlyBase IDs
    References (2)