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General Information
Symbol
TI{CRIMIC.TG4.0}
FlyBase ID
FBtp0129377
Feature type
DNA_segment
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Description
TI{CRIMIC.TG4.0} represents a DNA segment that has been inserted into the genome by homology directed repair using CRISPR/Cas9 in combination with a donor plasmid based on pM37_p0. The inserted TI{CRIMIC.TG4.0} DNA is not flanked by transposable element ends and consists of a recombination-mediated cassette exchange (RMCE) cassette flanked by a pair of inverted attP sites. Inside the attP sites is a cassette flanked by a pair of direct FRT sites. This in turn contains an intron phase 0 'Trojan GAL4' gene trap element (composed of a splice acceptor site followed by the T2A peptide, the GAL4 coding sequence and an SV40 polyadenylation signal), followed by the Avic\GFP3xP3.cLa marker allele. Integration of TI{CRIMIC.TG4.0} in a coding intron (with the same phase) of a native Drosophila gene of interest will result in the cassette behaving as a 'Trojan' exon: the splice acceptor site ensures that the T2A-GAL4 open reading frame is incorporated into the mRNA of the native Drosophila gene, while the T2A sequence truncates the native gene product and promotes the separate translation of the GAL4 open reading frame. Thus GAL4 should be expressed under the control of the regulatory sequences of the native Drosophila gene of interest in the resulting Trojan-CRIMIC GAL4 driver line. In addition, the presence of the inverted attP sites allows for the entire RMCE cassette sequence to be replaced with DNA from a compatible donor plasmid (where the sequence to be inserted is flanked by inverted attB sites) through recombination-mediated cassette exchange (RMCE) driven by the phiC31:int integrase.
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (169)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
TI{CRIMIC.TG4.0}
Secondary FlyBase IDs
    References (3)