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General Information
Symbol
P{10XUAS-ArchT-P2A-mRuby3.CAAX}
FlyBase ID
FBtp0133484
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
Construct components
Component allele HspTP009\ArchT10xUAS.Tag:HA
Product class / Tool use(s)
Description

10xUAS sequences drive expression of two coding regions that are separated by a P2A linker (this results in separate translation of each protein). The first coding sequence consists of HspTP009\ArchT (a light-gated outward proton pump) that is tagged at the C-terminal end with a Tag:HA epitope, a trafficking sequence, and an ER export sequence (represented in FlyBase by HspTP009\ArchT10xUAS.Tag:HA). The second coding sequence consists of mRuby3 that is tagged at the C-terminal end with a Tag:FLAG epitope and a Tag:CAAX(Unk) membrane targeting signal (represented in FlyBase by Equa\eqFP611mRuby3.10xUAS.Tag:FLAG,Tag:CAAX(Unk)).

Product class / Tool use(s)
Encoded product / tool
Description

10xUAS sequences drive expression of two coding regions that are separated by a P2A linker (this results in separate translation of each protein). The first coding sequence consists of HspTP009\ArchT (a light-gated outward proton pump) that is tagged at the C-terminal end with a Tag:HA epitope, a trafficking sequence, and an ER export sequence (represented in FlyBase by HspTP009\ArchT10xUAS.Tag:HA). The second coding sequence consists of mRuby3 that is tagged at the C-terminal end with a Tag:FLAG epitope and a Tag:CAAX(Unk) membrane targeting signal (represented in FlyBase by Equa\eqFP611mRuby3.10xUAS.Tag:FLAG,Tag:CAAX(Unk)).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Reference(s)
Molecular data

10xUAS sequences drive expression of two coding regions that are separated by a P2A linker (this results in separate translation of each protein). The first coding sequence consists of HspTP009\ArchT (a light-gated outward proton pump) that is tagged at the C-terminal end with a Tag:HA epitope, a trafficking sequence, and an ER export sequence (represented in FlyBase by HspTP009\ArchT10xUAS.Tag:HA). The second coding sequence consists of mRuby3 that is tagged at the C-terminal end with a Tag:FLAG epitope and a Tag:CAAX(Unk) membrane targeting signal (represented in FlyBase by Equa\eqFP611mRuby3.10xUAS.Tag:FLAG,Tag:CAAX(Unk)).

Reference(s)
Molecular data

10xUAS sequences drive expression of two coding regions that are separated by a P2A linker (this results in separate translation of each protein). The first coding sequence consists of HspTP009\ArchT (a light-gated outward proton pump) that is tagged at the C-terminal end with a Tag:HA epitope, a trafficking sequence, and an ER export sequence (represented in FlyBase by HspTP009\ArchT10xUAS.Tag:HA). The second coding sequence consists of mRuby3 that is tagged at the C-terminal end with a Tag:FLAG epitope and a Tag:CAAX(Unk) membrane targeting signal (represented in FlyBase by Equa\eqFP611mRuby3.10xUAS.Tag:FLAG,Tag:CAAX(Unk)).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{10XUAS-ArchT-P2A-mRuby3.CAAX}
UAS-ArchT-P2A-mRuby3CAAX
Secondary FlyBase IDs
    References (3)