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General Information
Symbol
PBac{U6:3-comt.gRNA}
FlyBase ID
FBtp0134088
Feature type
transgenic_transposon
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Tni\piggyBac
Construct components
Component allele comtU6:3.gRNA
Product class / Tool use(s)
Regulatory region(s)
Description

A snRNA:U6:96Ac promoter drives expression of two sgRNAs (AAAACGGTGGAGGTGCCCAG and GATGCCATTTGCAAGCAGCG), each of which target comt for knockout. The sgRNAs are expressed as a polycistronic cassette based on the tRNA-gRNA design of FBrf0233589.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Reference(s)
Molecular data

A snRNA:U6:96Ac promoter drives expression of two sgRNAs (AAAACGGTGGAGGTGCCCAG and GATGCCATTTGCAAGCAGCG), each of which target comt for knockout. The sgRNAs are expressed as a polycistronic cassette based on the tRNA-gRNA design of FBrf0233589.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{U6:3-comt.gRNA}
gRNA-Nsf1
Secondary FlyBase IDs
    References (2)