FB2025_01 , released February 20, 2025
Recombinant Construct: P{SAM.dCas9.GS05281}
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General Information
Symbol
P{SAM.dCas9.GS05281}
FlyBase ID
FBtp0137111
Feature type
transgenic_transposable_element
Component Allele(s)
SIFaSAM.dCas9.GS05281
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
MS2\MCPp65(h)::HSF1.10XUAS.flySAM2.0
Spyo\Cas9dCas9::VP64.10XUAS.flySAM2.0
v+t1.8
Component allele SIFaSAM.dCas9.GS05281
Transgenic product class
sgRNA
(Transgenic RNAi Project members, 2017-)
Component
Use(s)
Encoded product / tool
sgRNA-GS05281
Tagged with
Tag:MS2
Description

Constitutively expresses a single Tag:MS2-tagged sgRNA that targets sequence upstream of the transcription start site of SIFa. Forms one component of the three-component flySAM (synergistic activation mediator) system, which is used to produce effective transcriptional activation of a gene of interest.

(Transgenic RNAi Project members, 2017-)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
83620
y1 sc* v1 sev21; P{SAM.dCas9.GS05281}attP40
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{SAM.dCas9.GS05281}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)