Open Close
General Information
Symbol
PBac{UAS-ACOX1.N250S}
FlyBase ID
FBtp0142789
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele ACOX1N250S.UAS
Product class / Tool use(s)
Description

UASt regulatory sequences drive expression of the full-length ACOX1 open reading frame (ORF) (from the GH07485 cDNA clone), mutated to carry a N250S amino acid substitution. This change is equivalent to a N237S change in the human ACOX1 gene, a variant identified in patients with progressive ataxia and hearing loss. The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Component Alleles
Reference(s)
Molecular data

UASt regulatory sequences drive expression of the full-length ACOX1 open reading frame (ORF) (from the GH07485 cDNA clone), mutated to carry a N250S amino acid substitution. This change is equivalent to a N237S change in the human ACOX1 gene, a variant identified in patients with progressive ataxia and hearing loss. The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{UAS-ACOX1.N250S}
UAS-dACOX1N250S
Secondary FlyBase IDs
    References (2)