A synthetic neuroblast enhancer ('DNE') that comprises a 607bp region starting 899bp 5' of the dpn transcription start site fused via a 10bp linker to a nerfin-1 enhancer that carries two nucleotide substitutions that expand the range of expression in neuroblasts, is followed by an FRT cassette that contains two coding regions separated by a T2A linker (this results in separate translation of each protein). The first coding region encodes the FLP recombinase (represented in FlyBase by Scer\FLP1^{DNE.FRTerminator}), the second coding region encodes the creAB::NrdJ-1N split recombinase component (represented in FlyBase by P1\cre^{AB.DNE.FRTerminator}). The transgene terminates its own expression: the DNE enhancer initially drives expression of both coding regions in early neuroblasts, and the FLP recombinase produced then excises the entire coding cassette, preventing further expression of both FLP and creAB::NrdJ-1N, limiting expression to early neuroblasts.

A synthetic neuroblast enhancer ('DNE') that comprises a 607bp region starting 899bp 5' of the dpn transcription start site fused via a 10bp linker to a nerfin-1 enhancer that carries two nucleotide substitutions that expand the range of expression in neuroblasts, is followed by an FRT cassette that contains two coding regions separated by a T2A linker (this results in separate translation of each protein). The first coding region encodes the FLP recombinase (represented in FlyBase by Scer\FLP1^{DNE.FRTerminator}), the second coding region encodes the creAB::NrdJ-1N split recombinase component (represented in FlyBase by P1\cre^{AB.DNE.FRTerminator}). The transgene terminates its own expression: the DNE enhancer initially drives expression of both coding regions in early neuroblasts, and the FLP recombinase produced then excises the entire coding cassette, preventing further expression of both FLP and creAB::NrdJ-1N, limiting expression to early neuroblasts.

A synthetic neuroblast enhancer ('DNE') that comprises a 607bp region starting 899bp 5' of the dpn transcription start site fused via a 10bp linker to a nerfin-1 enhancer that carries two nucleotide substitutions that expand the range of expression in neuroblasts, is followed by an FRT cassette that contains two coding regions separated by a T2A linker (this results in separate translation of each protein). The first coding region encodes the FLP recombinase (represented in FlyBase by Scer\FLP1^{DNE.FRTerminator}), the second coding region encodes the creAB::NrdJ-1N split recombinase component (represented in FlyBase by P1\cre^{AB.DNE.FRTerminator}). The transgene terminates its own expression: the DNE enhancer initially drives expression of both coding regions in early neuroblasts, and the FLP recombinase produced then excises the entire coding cassette, preventing further expression of both FLP and creAB::NrdJ-1N, limiting expression to early neuroblasts.

A synthetic neuroblast enhancer ('DNE') that comprises a 607bp region starting 899bp 5' of the dpn transcription start site fused via a 10bp linker to a nerfin-1 enhancer that carries two nucleotide substitutions that expand the range of expression in neuroblasts, is followed by an FRT cassette that contains two coding regions separated by a T2A linker (this results in separate translation of each protein). The first coding region encodes the FLP recombinase (represented in FlyBase by Scer\FLP1^{DNE.FRTerminator}), the second coding region encodes the creAB::NrdJ-1N split recombinase component (represented in FlyBase by P1\cre^{AB.DNE.FRTerminator}). The transgene terminates its own expression: the DNE enhancer initially drives expression of both coding regions in early neuroblasts, and the FLP recombinase produced then excises the entire coding cassette, preventing further expression of both FLP and creAB::NrdJ-1N, limiting expression to early neuroblasts.