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General Information
Symbol
P{DNE-FRTerminator}
FlyBase ID
FBtp0142991
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele Scer\FLP1DNE.FRTerminator
Product class / Tool use(s)
Encoded product / tool
Description

A synthetic neuroblast enhancer ('DNE') that comprises a 607bp region starting 899bp 5' of the dpn transcription start site fused via a 10bp linker to a nerfin-1 enhancer that carries two nucleotide substitutions that expand the range of expression in neuroblasts, is followed by an FRT cassette that contains two coding regions separated by a T2A linker (this results in separate translation of each protein). The first coding region encodes the FLP recombinase (represented in FlyBase by Scer\FLP1DNE.FRTerminator), the second coding region encodes the creAB::NrdJ-1N split recombinase component (represented in FlyBase by P1\creAB.DNE.FRTerminator). The transgene terminates its own expression: the DNE enhancer initially drives expression of both coding regions in early neuroblasts, and the FLP recombinase produced then excises the entire coding cassette, preventing further expression of both FLP and creAB::NrdJ-1N, limiting expression to early neuroblasts.

Component allele P1\creAB.DNE.FRTerminator
Product class / Tool use(s)
Encoded product / tool
Description

A synthetic neuroblast enhancer ('DNE') that comprises a 607bp region starting 899bp 5' of the dpn transcription start site fused via a 10bp linker to a nerfin-1 enhancer that carries two nucleotide substitutions that expand the range of expression in neuroblasts, is followed by an FRT cassette that contains two coding regions separated by a T2A linker (this results in separate translation of each protein). The first coding region encodes the FLP recombinase (represented in FlyBase by Scer\FLP1DNE.FRTerminator), the second coding region encodes the creAB::NrdJ-1N split recombinase component (represented in FlyBase by P1\creAB.DNE.FRTerminator). The transgene terminates its own expression: the DNE enhancer initially drives expression of both coding regions in early neuroblasts, and the FLP recombinase produced then excises the entire coding cassette, preventing further expression of both FLP and creAB::NrdJ-1N, limiting expression to early neuroblasts.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Reference(s)
Molecular data

A synthetic neuroblast enhancer ('DNE') that comprises a 607bp region starting 899bp 5' of the dpn transcription start site fused via a 10bp linker to a nerfin-1 enhancer that carries two nucleotide substitutions that expand the range of expression in neuroblasts, is followed by an FRT cassette that contains two coding regions separated by a T2A linker (this results in separate translation of each protein). The first coding region encodes the FLP recombinase (represented in FlyBase by Scer\FLP1DNE.FRTerminator), the second coding region encodes the creAB::NrdJ-1N split recombinase component (represented in FlyBase by P1\creAB.DNE.FRTerminator). The transgene terminates its own expression: the DNE enhancer initially drives expression of both coding regions in early neuroblasts, and the FLP recombinase produced then excises the entire coding cassette, preventing further expression of both FLP and creAB::NrdJ-1N, limiting expression to early neuroblasts.

Reference(s)
Molecular data

A synthetic neuroblast enhancer ('DNE') that comprises a 607bp region starting 899bp 5' of the dpn transcription start site fused via a 10bp linker to a nerfin-1 enhancer that carries two nucleotide substitutions that expand the range of expression in neuroblasts, is followed by an FRT cassette that contains two coding regions separated by a T2A linker (this results in separate translation of each protein). The first coding region encodes the FLP recombinase (represented in FlyBase by Scer\FLP1DNE.FRTerminator), the second coding region encodes the creAB::NrdJ-1N split recombinase component (represented in FlyBase by P1\creAB.DNE.FRTerminator). The transgene terminates its own expression: the DNE enhancer initially drives expression of both coding regions in early neuroblasts, and the FLP recombinase produced then excises the entire coding cassette, preventing further expression of both FLP and creAB::NrdJ-1N, limiting expression to early neuroblasts.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
DNE-FRTerminator
FRTerminator
P{DNE-FRTerminator}
Secondary FlyBase IDs
    References (2)