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General Information
Symbol
P{LexAop2-TEV.CD2.P10}
FlyBase ID
FBtp0145454
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Product class / Tool use(s)
Description

The Scer\FLP1 cassette present in P{LexAop2(RSRT.Flp1)TEV.CD2.P10} has been removed via FLP, leaving behind a single RSRT site (that is innocuous). As a result, lexAop (LexAop2) regulates expression of TEV\NIa followed by the extracellular portion of a transmembrane protein (Tag:M(rCd2)). The N-terminus has the C. elegans PAT3 signal peptide sequence for endoplasmic reticulum targeting, while the C-terminus has the Kir2.1 ER export signal to improve trafficking to the plasma membrane, followed by the P10 3' UTR. Tag:MYC has been introduced in between the TEV\NIa and Tag:M(rCd2) sequence. (GenBank MN658787.) Used in the first (release) step of the BacTrace system.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Component Alleles
Reference(s)
Molecular data

The Scer\FLP1 cassette present in P{LexAop2(RSRT.Flp1)TEV.CD2.P10} has been removed via FLP, leaving behind a single RSRT site (that is innocuous). As a result, lexAop (LexAop2) regulates expression of TEV\NIa followed by the extracellular portion of a transmembrane protein (Tag:M(rCd2)). The N-terminus has the C. elegans PAT3 signal peptide sequence for endoplasmic reticulum targeting, while the C-terminus has the Kir2.1 ER export signal to improve trafficking to the plasma membrane, followed by the P10 3' UTR. Tag:MYC has been introduced in between the TEV\NIa and Tag:M(rCd2) sequence. (GenBank MN658787.) Used in the first (release) step of the BacTrace system.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
LexAop2-RSRT-TEV::CD2-P10
P{LexAop2-TEV.CD2.P10}
Secondary FlyBase IDs
    References (2)