FB2025_01 , released February 20, 2025
Recombinant Construct: P{UAS-mCh-T2A-UGG-321-T2A-GFP}
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General Information
Symbol
P{UAS-mCh-T2A-UGG-321-T2A-GFP}
FlyBase ID
FBtp0147384
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

UAS regulatory sequences drive expression of a reporter in which two fluorescent proteins (mCherry and GFP) are separated by a kel gene test fragment (that extends from 84 nucleotides 5' to 321 nucleotides 3' relative to the UGA kel ORF1 stop codon and in which the UGA codon has been mutated to an in-frame UGG) which is flanked on each side by a T2A sequence. Designed to be used a control in an assay to test the ability of the kel fragment to mediate stop codon readthrough; readthrough efficiency can be calculated from the ratio of GFP produced by P{UAS-mCh-T2A-UGA-321-T2A-GFP} relative to this control construct.

(Hudson et al., 2021)
Construct components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
GFP
mCherry
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{UAS-mCh-T2A-UGG-321-T2A-GFP}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)