TI{KozakGAL4} represents a DNA segment that has been inserted into the genome by homology directed repair using CRISPR/Cas9 in combination with a donor homology construct based on pM37_KozakGAL4. The inserted TI{KozakGAL4} DNA is not flanked by transposable element ends and contains an artificial exon composed of 1. a translation initiation site (CAAA Kozak sequence), the GAL4 coding sequence and a polyA signal and 2. an FRT cassette containing a Avic\GFP3xP3.cLa marker. The TI{KozakGAL4} DNA segment is typically inserted into a gene of interest using a strategy that replaces the coding region of the targeted gene with the TI{KozakGAL4} sequence, thus generating a simultaneous knock-out of the targeted gene and a knock-in of GAL4 sequence that is expected to be expressed under the control of the endogenous regulatory sequences of the targeted gene.
