TI{SA-KozakGAL4} represents a DNA segment that has been inserted into the genome by homology directed repair using CRISPR/Cas9 in combination with a donor homology construct based on pM37_SA_KozakGAL4. The inserted TI{SA-KozakGAL4} DNA is not flanked by transposable element ends and contains an artificial exon comprising 1. a gene trap element composed of a splice acceptor site, a translation initiation site (CAAA Kozak sequence), the GAL4 coding sequence and a polyA signal and 2. an FRT cassette containing a Avic\GFP3xP3.cLa marker. The presence of the splice acceptor site and Kozak sequence in the gene trap element means that when the TI{SA-KozakGAL4} sequence is inserted into an intron in the 5'UTR of an endogenous locus, the GAL4 driver is expected to be expressed under the control of the regulatory sequences of the trapped gene.
