FB2025_01 , released February 20, 2025
Recombinant Construct: P{UASp-Acly.H772A.3xHA}
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General Information
Symbol
P{UASp-Acly.H772A.3xHA}
FlyBase ID
FBtp0165212
Feature type
transgenic_transposable_element
Component Allele(s)
AclyH772A.UASp.Tag:HA
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele AclyH772A.UASp.Tag:HA
Transgenic product class
inactive_catalytic_site
(François et al., 2023)
missense_variant
(François et al., 2023)
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Acly
Tagged with
Tag:HA
Description

UASp regulates expression of Acly cDNA (FBpp0289823) containing a catalytically-dead H772>A variant, and tagged with 3xTag:HA before the stop codon. The sequence targeted by P{TRiP.HMC06049} is mutated to make the UAS line resistant to the shRNA (CACGACCTATGTA- GACCTGTA > TACAACGTACGTGGATTTATA, 8 mutations introduced without affecting the Acly protein sequence).

(François et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UASp-Acly.H772A.3xHA}
(FlyBase, 1992-)
UASp-dACLYH772>A
(François et al., 2023)
Secondary FlyBase IDs
    References (2)