FB2025_01 , released February 20, 2025
Recombinant Construct: PBac{UAS-hDOT1L.R292C.HA}
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General Information
Symbol
PBac{UAS-hDOT1L.R292C.HA}
FlyBase ID
FBtp0165626
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\DOT1LR292C.UAS.Tag:HA
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Hsap\DOT1LR292C.UAS.Tag:HA
Transgenic product class
missense_variant
(Nil et al., 2023)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\DOT1L
Tagged with
Tag:HA
Also carries
FRT2
FRT5
loxP
Description

UASt regulatory sequences drive expression of the full-length Hsap\DOT1L open reading frame (corresponds to GB:NM_032482.3), mutated to carry a p.Arg292Cys change .The coding sequence is tagged at the C-terminal end with three copies of Tag:HA. The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Nil et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{UAS-hDOT1L.R292C.HA}
(FlyBase, 1992-)
UAS-DOT1L p.Arg292Cys
(Nil et al., 2023)
Secondary FlyBase IDs
    References (2)