FB2025_05 , released December 11, 2025
Recombinant Construct: P{UAS-Wnt4+wntD.pCFD6}
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General Information
Symbol
P{UAS-Wnt4+wntD.pCFD6}
FlyBase ID
FBtp0170747
Feature type
transgenic_transposable_element
Component Allele(s)
Wnt4UAS.Wnt4+wntD.pCFD6
wntDUAS.Wnt4+wntD.pCFD6
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Wnt4UAS.Wnt4+wntD.pCFD6
Transgenic product class
sgRNA
(Transgenic RNAi Project, 2024.8.21)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Wnt4
Description

UAS regulatory sequences drive expression of four sgRNAs; two target Wnt4 (sgRNA sequences TGTTCAGATTGTGATTGCTCTGG and TCGAGTGTACCCTCGGTGATTGG) and two target wntD (sgRNA sequences GTGTACTGGTAGTAGCTCATGGG and CATGGGTATTACGAGCACTCTGG). The sgRNAs are expressed as a single polycistronic tRNA-gRNA cassette.

(Transgenic RNAi Project, 2024.8.21)
Component allele wntDUAS.Wnt4+wntD.pCFD6
Transgenic product class
sgRNA
(Transgenic RNAi Project, 2024.8.21)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
wntD
Description

UAS regulatory sequences drive expression of four sgRNAs; two target Wnt4 (sgRNA sequences TGTTCAGATTGTGATTGCTCTGG and TCGAGTGTACCCTCGGTGATTGG) and two target wntD (sgRNA sequences GTGTACTGGTAGTAGCTCATGGG and CATGGGTATTACGAGCACTCTGG). The sgRNAs are expressed as a single polycistronic tRNA-gRNA cassette.

(Transgenic RNAi Project, 2024.8.21)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
605669
w1118; P{UAS-Wnt4+wntD.pCFD6}attP40/CyO
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Wnt4+wntD.pCFD6}
(FlyBase, 1992-)
wnt4+wntD sgRNA
(Ewen-Campen and Perrimon, 2024)
Secondary FlyBase IDs
    References (4)