FB2025_01 , released February 20, 2025
Recombinant Construct: P{3.8S-Y}
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General Information
Symbol
P{3.8S-Y}
FlyBase ID
FBtp0171971
Feature type
transgenic_transposable_element
Component Allele(s)
Cp15ch1
Cp18ch1
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Description

Transgenic construct used to study whether the gypsy insulator can protect DNA replication from position effects. Contains (5' to 3'): a gypsy insulator - the y+mDint2 marker - a 3.8kb SalI genomic fragment from the third chromosome chorion gene cluster (this includes the ACE3 amplification control element, the Cp18 transcription unit, the Ori-β origin of replication and the Cp15 transcription unit) - a gypsy insulator.

(Lu and Tower, 1997)
Construct components
Component
Use(s)
Marker(s)
y+mDint2
Component allele Cp15ch1
Transgenic product class
Component
Use(s)
Encoded product / tool
Cp15
Description

Construct: 3.8kb SalI genomic fragment containing the Cp15 and Cp18 transcripts.

(Rubin and Spradling, 1982)
Component allele Cp18ch1
Transgenic product class
Component
Use(s)
Encoded product / tool
Cp18
Description

Construct: 3.8kb SalI genomic fragment containing the Cp15 and Cp18 transcripts.

(Rubin and Spradling, 1982)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

construct_comment: P{Yes-3.BS} contains (5\' to 3\'): gypsy\su(Hw)BR430 fused to the the y gene, fused to a genomic fragment containing ACE3 (+ some 5\' flanking sequence) , Cp1, Ori66Dβ and Cp15, fused at the 3\' end to an inverted gypsy\su(Hw)BR430.

(Zhang and Tower, 2004)

P{Yes-3.BS} contains (5\' to 3\'): gypsy\su(Hw)BR430 fused to the the y gene, fused to a genomic fragment containing ACE3 (+ some 5\' flanking sequence) , Cp1, Ori66Dβ and Cp15, fused at the 3\' end to an inverted gypsy\su(Hw)BR430.

(Zhang and Tower, 2004)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{3.8S-Y}
(FlyBase, 1996-)
P{Yes-3.BS}
(FlyBase, 1996-)
Yes-3.8S
(Zhang and Tower, 2004)
Secondary FlyBase IDs
  • FBtp0011311
  • FBtp0020624
References (5)