Transgenic construct used to study whether the gypsy insulator can protect DNA replication from position effects. Contains (5' to 3'): a gypsy insulator - the y+mDint2 marker - a 3.8kb SalI genomic fragment from the third chromosome chorion gene cluster (this includes the ACE3 amplification control element, the Cp18 transcription unit, the Ori-β origin of replication and the Cp15 transcription unit) - a gypsy insulator.
construct_comment: P{Yes-3.BS} contains (5\' to 3\'): gypsy\su(Hw)BR430 fused to the the y gene, fused to a genomic fragment containing ACE3 (+ some 5\' flanking sequence) , Cp1, Ori66Dβ and Cp15, fused at the 3\' end to an inverted gypsy\su(Hw)BR430.
P{Yes-3.BS} contains (5\' to 3\'): gypsy\su(Hw)BR430 fused to the the y gene, fused to a genomic fragment containing ACE3 (+ some 5\' flanking sequence) , Cp1, Ori66Dβ and Cp15, fused at the 3\' end to an inverted gypsy\su(Hw)BR430.