Since the 2007 publication of the 12 species genomes, the genomes of nine additional species have been sequenced, bringing the total to 21. The UCSD stock center has the strains of the newly sequenced species in addition to a number of other stocks of each of these. Please visit the DSSC website for more information:

https://stockcenter.ucsd.edu/info/welcome.php

This is the ONLY call for submissions for the 2012 Drosophila Image Award. The award will be presented at the upcoming Drosophila Research Conference in Chicago on March 8th, 2012. Presence at the meeting is not required to enter or win an Award.

The Image Award recognizes the important role that compelling images have played in Drosophila research. To encourage and celebrate this role, finalist images will be displayed throughout the conference, and the winner will be presented with a plaque.

The Award will be given to the most striking image that clearly conveys a point of important biological information in Drosophila research. All images that have been or will be published in a primary research journal in 2011 are eligible. Submissions can be made electronically to imageawardlists.berkeley.edu. Please see http://www.Drosophila-images.org for more details and complete instructions for submission, as well as for images of past winners.

FlyBase 2012 Release Schedule

The following are the tentative dates of FlyBase releases in 2012. There will be 6 releases this year.

• FB2012_01 - January 6^th
• FB2012_02 - March 2^nd
• FB2012_03 - May 4^th
• FB2012_04 - July 6^th
• FB2012_05 - September 7^th
• FB2012_06 - November 2^nd

Please feel free to Contact us with any questions or comments.

On behalf of the entire Drosophila research community, FlyBase congratulates Nobel Laureate Jules Hoffmann. Professor Hoffmann was honored with a share of the Nobel Prize in Physiology or Medicine 2011 for his pioneering work on the innate immune system using Drosophila. Hoffmann and colleagues are in particular cited for having provided the first evidence that Toll receptors mediate the innate immune response.

The coordinates for the 5-state chromatin domains of Filion et al. (2010) were incorrectly converted by FlyBase from release 4 of the D. melanogaster genome scaffold to the current release 5 build. The authors have informed us that the data, generated on release-4-based microarrays, were already converted into release 5 coordinates upon deposition in the GEO data repository. The coordinates for the GBrowse chromatin domains track are now correctly displayed.

The following is a reproduction of an announcement by Developmental Dynamics:

Call for Papers for a Special Issue in DEVELOPMENTAL DYNAMICS on “Drosophila as a Model for Understanding Development and Disease”

We are pleased to announce a call for papers for a Special Issue of Developmental Dynamics celebrating the importance of research on Drosophila in development and disease. The issue will be guest-edited by Ken Irvine (irvinewaksman.rutgers.edu), Rutgers University, and Amit Singh (amit.singhnotes.udayton.edu ), University of Dayton.

Drosophila investigators are invited to submit papers to Developmental Dynamics for a special issue of the journal to be published in late 2011 or early 2012. Papers should be submitted no later than June 15, 2011, for consideration for the special issue. Research articles exploring mechanisms of development, Technique articles describing new techniques of broad impact, or Disease Connection articles describing novel models/preparations/approaches for understanding the developmental basis of disease are welcomed. Ideas for appropriate Reviews articles are also encouraged (contact the Reviews Editor, John F. Fallon: jffallonwisc.edu). All articles undergo thorough peer review to determine their merits for publication.

Developmental Dynamics is committed to publishing papers of central importance to the developmental biology community. All special issue articles are open access immediately upon publication, allowing your work to be disseminated widely throughout the scientific community. There are no page or color charges, and Developmental Dynamics is fully compliant with the open access policies of NIH, HHMI, and the Wellcome Trust.

Manuscripts should be submitted online at: http://mc.manuscriptcentral.com/dvdy-wiley

Author Guidelines can be found at:
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0177/homepage/ForAuthors.html

We look forward to receiving your submissions.

Best wishes,
Gary C. Schoenwolf, Ph.D.
Editor-in-Chief, Developmental Dynamics

We sadly announce that Dr. Victoria Finnerty, Professor of Biology at Emory University, has passed away at the age of 73. She remained a fully active faculty member until the end of last year. She was in Chicago with her son and his family during the last few months. After her graduate and postdoctoral studies with Art Chovnick at the University of Connecticut, Vickie came to Emory in 1976 where she maintained an active lab throughout her career. Her early seminal work focused on the use of high resolution recombinational analysis of the maroon-like gene to understand gene structure in Drosophila. Her interests then shifted to understanding the molecular function of the maroon-like gene in the production of a molybdenum-containing cofactor required for the activity of xanthine dehydrogenase (rosy) and other enzymes. Vickie was well-known as a gifted teacher and mentor, and during the last several years of her career she focused most of her efforts on undergraduate education in the Emory Biology Department. Her kindness, warmth, dedication and even-tempered manner will be greatly missed by her many friends in the Drosophila community. She was loved by all who knew her.

Respectfully,
Barry Yedvobnick, Janis O'Donnell & Bill Gelbart

FlyBase is pleased to note the publication on December 22, 2010 of an extremely important set of papers from the modENCODE project, including two integrative papers (one on Drosophila melanogaster and the other on Caenorhabditis elegans DNA elements) as well as a series of companion papers. The complete list of papers is available through the modENCODE website (http://blog.modencode.org/papers) and the major impact of the work is summarized in a press release from NHGRI (http://www.genome.gov/27542795).

On behalf of the Drosophila research community, we wish to acknowledge and thank NHGRI for its generous support of this major community resource project. FlyBase is in the process of working with the modENCODE project to incorporate the summary results of these studies into FlyBase. As data sets are incorporated, we will post notices describing the new data on FlyBase.

FlyBase 2011 Release Schedule

The following are the tentative dates of FlyBase releases in 2011. There will be 10 releases this year with no releases in August and December.

• FB2011_01 - January 21st
• FB2011_02 - February 18th
• FB2011_03 - March 18th March 21st
• FB2011_04 - April 22nd
• FB2011_05 - May 27th
• FB2011_06 - June 24th
• FB2011_07 - July 22nd
• FB2011_08 - September 2nd
• FB2011_09 - October 7th October 10th
• FB2011_10 - November 11th November 18th

Please feel free to Contact us with any questions or comments.

FlyBase has added support for Zotero to our reference reports and any search result that returns references.

To utilize this functionality you need to use Firefox and have Zotero installed. After you have satisfied those requirements you should see a Zotero icon in the Firefox location bar any time you view a FlyBase reference report or a search result. This icon indicates that you can import the current citation or list of citations into your Zotero database by clicking on it.

For more information about Zotero please see http://www.zotero.org/.

If you have a question or comment about FlyBase please feel free to Contact FlyBase.

Dear Drosophilist,

The Duplication Consortium, comprised of members of the Kaufman, Bellen and Hoskins labs, has used and is using the 83 kb P[acman] libraries (Nat Methods (2009) 6:431-434) to generate a set of duplications to cover the X chromosome. The initial set of these duplications are now available at the Bloomington Drosophila Stock Center and will soon appear on the GBrowse maps at FlyBase.

The specific coverage of each of these duplications can be viewed on the Pacman Resources website at http://pacmanfly.org/dups.php as well as on the BDSC web site http://fly.bio.indiana.edu/Browse/dp/DC-Dps.php. As more duplication lines are made they will be deposited at the BDSC and they will be added to these sites. Our goal is to provide, as far as is possible, complete coverage of the euchromatic X Chromosome. We hope those of you who may need them will find them useful. Any feedback about the stocks, will be appreciated.

Sincerely,

The Duplication Consortium

We announce the resumption of the Fly-TILL reverse-genetics service. Fly-TILL first opened in 2005 as a non-profit service supported by user fees to discover point mutations in genes of interest for the Drosophila community. Fly-TILL uses a mismatch detection strategy developed at the Fred Hutchinson Center with NSF funding, and introduced as a service for the Arabidopsis community in 2002. The method was later applied to screening balanced Chromosome 2 and 3 lines that had been produced and maintained by Charles Zuker and colleagues at UCSD. About 200 allelic series averaging ~9 mutations per order were discovered and reported to users by the Seattle TILLING Project at the University of Washington and the Hutch (Cooper, Greene, et al. Genetics 180:661-7, 2008; Cooper, Till, et al. Fly 2:300-2, 2008), and mutant lines were distributed by the Zuker lab. Fly-TILL was suspended at the end of 2008 when the Zuker lines were no longer maintained. However, new EMS-mutagenized populations of balanced Chromosome 2 and 3 lines have been established by the Hawley group at the Stowers Institute, and DNAs have been prepared and tested for screening in collaboration with the Henikoff group at the Hutch.

We are pleased to report that the Hawley lines are more densely mutagenized than were the Zuker lines, which will allow us to maintain and screen fewer lines at lower cost to deliver a suitable allelic series. In addition, no culling of lethals was carried out on the Hawley lines, as was the case for the Zuker lines, so that a broad range of EMS-induced mutations should be present. Based on our screening and quality control tests thus far, we expect that the average allelic series will yield 7-8 mutations, of which 50% will be missense, 5% truncation and 45% silent. Because this is random mutagenesis, we cannot predict the mutation frequency and distribution of any single allelic series. A user fee of $1750 per allelic series will cover the cost of screening ~1500 DNA samples derived from the Chromosome 2 or 3 lines at the Hutch, and stocks will be distributed by the Hawley lab upon request. Former Fly-TILL users who wish to have the Hawley lines screened for same amplicons used on the Zuker lines will be billed $1500 per allelic series.

We anticipate reopening Fly-TILL service on Monday June 14, 2010, at which time orders will be processed on a first-come, first-served basis. Orders placed before then will be put in the queue, and orders in the queue will be billed and processed when Fly-TILL reopens, capacity permitting. Please note that we will not be able to maintain these beyond July 1, 2011. To learn more about Fly-TILL or to put orders in the queue, go to http://tilling.fhcrc.org/fly/. The Web site describes how TILLING works, what Fly-TILL expects to deliver, answers to frequently asked questions and user policies.

FlyBase is currently participating in the Gene Ontology Consortium's Reference Genome Project, a collaborative project to comprehensively annotate gene function for twelve 'Reference Genomes': D. melanogaster, H. sapiens, M. musculus, R. norvegicus, G. gallus, D. rerio, D. discoideum, A. thaliana, C. elegans, S. cerevisiae, S. pombe and E. coli. The Reference Genome project aims to improve the breadth and depth of GO annotation for participating genomes as well as provide reference annotations for newly sequenced or previously unannotated genomes.

For more information, please see the GO Reference Genome Annotation Project and this paper: PLoS Comput Biol 2009 5(7):e1000431.

An update to the Drosophila melanogaster annotations was published by NCBI on December 18, 2009. This update is reflected in GenBank, RefSeq, and Entrez Gene records. This GenBank release corresponds to release 5.22 of the D. melanogaster genome annotation except for the few changes noted below. We plan to continue to submit D. melanogaster annotation updates to NCBI approximately twice a year. Exceptions to this timetable will occur when the assembly of the reference D. melanogaster genome is updated and annotations are migrated onto the new assembly.

Differences between the December 18, 2009 GenBank annotations and those in release 5.22 of FlyBase:

1. Annotation CG40626 is not present in the GenBank submission and was subsequently deleted in annotation release 5.23 of Dmel in FlyBase as it corresponds to a redundant fragment in the unordered scaffold U.
2. Annotations CG15364, CG4040, CG33224 are not present in the GenBank submission as they were present in release 5.22 only due to an incomplete merge of these annotations into CG42388 that occurred in a previous release.
3. The mRNA and protein genome location for CG41561-RA and CG41561-PA were included in the GenBank submission and in subsequent FlyBase releases as it was unintentionally deleted from release 5.22.
4. EcR-RF and EcR-PF were renamed to EcR-RG and EcR-PG, respectively in the GenBank submission and subsequent FlyBase releases as the RF/PF suffix had been previously used for different isoforms in a previous GenBank submission.
5. CG32573-RB and CG32573-PB were renamed to CG32573-RC and CG32573-PC, respectively in the GenBank submission and subsequent FlyBase releases as the RF/PF suffix had been previously used for a different isoform in a previous GenBank submission.

FlyBase 2010 Release Schedule

The following are the tentative dates of FlyBase releases in 2010. There will be 9 releases this year with no releases in July, August, and December.

• FB2010_01 - January 22nd
• FB2010_02 - February 19th
• FB2010_03 - March 19th
• FB2010_04 - April 23rd
• FB2010_05 - May 28th
• FB2010_06 - June 25th
• FB2010_07 - July 23rd- Canceled ¹
• FB2010_07 - September 3rd
• FB2010_08 - October 8th - Delayed until October 13th
• FB2010_09 - November 12th - Delayed until November 19th

Please feel free to Contact us with any questions or comments.

1. The July 23rd release has been canceled so that we can focus on a few new features and data types.

From this point forward FlyBase will be providing Apollo viewable annotation data in chado-xml format only. The latest version of Apollo has been modified to retrieve annotation data via the web in this format. Therefore, we recommend that you upgrade to the latest available version of Apollo

We will no longer provide annotation data in GAME-XML format and while Apollo is still able to load and view existing GAME-XML data the data files must be available on the local machine as web retrieval of GAME files is no longer supported.

Please be aware that when Apollo is retrieving annotation data from the web it is obtaining precomputed chunks of xml. While the pieces have been generated to not span any gene models retrieval by sequence range may not result in the exact sequence requested being loaded into Apollo.

Please use the contact FlyBase form to send questions and requests for help to FlyBase.

FlyBase offers direct "linkouts" from our gene report pages to other web-based Drosophila data services. The FlyBase Consortium wishes to thank the organizers and developers of these data sets for helping us provide a great deal of valuable information on Drosophila genes and genomes.

• BDGP in situ Gene Expression Database (http://toy.lbl.gov:8888/cgi-bin/ex/insitu.pl)
• Drosophila melanogaster Exon Database (http://proline.bic.nus.edu.sg/dedb/index.html)
• Drosophila RNAi Screening Center (http://www.flyrnai.org/)
• FLIGHT - Integrating Genomic and High-Throughput data (http://flight.licr.org/)
• Fly GRID Interaction Data (http://biodata.mshri.on.ca:80/fly_grid/servlet/SearchPage)
• FlyMine - integrated genomics and proteomics (http://www.flymine.org/)
• NCBI Gene Expression Omnibus (GEO) (http://www.ncbi.nlm.nih.gov/projects/geo/)
• Heidelberg Database for RNAi Phenotypes (http://www.dkfz-heidelberg.de/signaling/ernai/ernai.html)
• Hybrigenics Drosophila PIMRider (http://pim.hybrigenics.com/pimriderext/droso/index.html)
• Yale Developmental Gene Expression (http://genome.med.yale.edu/Lifecycle/)
• InParanoid computed orthology calls (http://inparanoid.cgb.ki.se/index.html)
• PANTHER ClassificationSystem (http://www.pantherdb.org/)

In addition to these links FlyBase also provide direct links to the Drosophila records in the major international nucleotide, protein and bibliographic databases.

Szeged Stocks Transferred To Kyoto

Following the closure of the Szeged stock centre in June 2009 the majority of the stocks have now been transferred to the DGRC in Kyoto (http://kyotofly.kit.jp/stocks).

Among the stocks now available are the complete set of molecularly-defined ED deletions and 1500 of the RS3 and RS5 lines, from the DrosDel consortium (http://www.drosdel.org.uk/). More RS lines will become available soon.

(Note: the RS insertions carry FRT sites which, for example, can be used as docking sites for FLP-mediated transgene remobilisation, using the split white + marker strategy. For review see Venken and Bellen (2007) Development 134:3571-3584.)

The RS lines are listed here:

• http://kyotofly.kit.jp/cgi-bin/stocks/index.cgi
• http://kyotofly.kit.jp/cgi-bin/stocks/search_res_list.cgi?DB_NUM=1&PREDEF=Szeged

• http://kyotofly.kit.jp/cgi-bin/stocks/data_search.cgi#DrosDel
• http://kyotofly.kit.jp/cgi-bin/stocks/search_res_list.cgi?DB_NUM=1&PREDEF=DrosDel

Release of a first draft of the Drosophila santomea genome sequence (v1.0)

D. santomea was first described by Lachaise et al (2000) as a new melanogaster-group sister species endemic to the island of São Tomé off the coast of West Africa. D. santomea is most closely related to D. yakuba and the two species diverged ~0.5 Mya (Cariou et al. 2001; Llopart et al. 2005; Bachtrog et al. 2006). The species has a number of derived characters relative to D. yakuba, including highly reduced pigmentation and mating and temperature preferences, making it fertile ground for studies of the evolution of novel characters (Llopart et al. 2002; Coyne et al. 2004; Carbone et al. 2005; Llopart et al. 2005; Mas and Jallon 2005; Moehring et al. 2006; Jeong et al. 2008; Matute and Coyne 2009). The species is only partially reproductively isolated from D. yakuba, facilitating the genetic dissection of the factors underlying reproductive isolation and other derived phenotypic traits of interest.

The current draft genome represents a bwa (http://bio-bwa.sourceforge.net/bwa.shtml) assembly of 65.9 million 54 bp Illumina sequence reads to the D. yakuba reference genome sequence (release 1.3) yielding an average coverage of ~10X. Further updates are expected soon, including higher coverage with paired-end reads and a de novo assembly in collaboration with Mike Eisen at UC Berkeley.

This first release can be downloaded at this website:

http://genomics.princeton.edu/AndolfattoLab/Links.html

We hope this will stimulate evolutionary genetic research on D. santomea and other Drosophila species - enjoy!

• Peter Andolfatto
• Tina Hu
• Kevin Thornton