FB2014_05, released September 9th, 2014

A Database of Drosophila Genes & Genomes

New tracks in GBrowse: transcription factor binding sites

FlyBase has incorporated new transcription factor binding site (TFBS) datasets as tracks in GBrowse. As part of the modENCODE project, Nègre et al., have generated a cis-regulatory map of the D. melanogaster genome. Combining genome-wide chromatin immunoprecipitation experiments from both the modENCODE consortium and the Berkeley Drosophila Transcription Network Project, binding sites have been identified for 41 different transcription factors. These binding sites are separated into five different GBrowse tracks according to the class of bound transcription factor.

transcription factor binding sites GBrowse tracks screenshot

One key finding of this work is the extensive overlap in the binding profiles of many transcription factors. This aspect of the cis-regulatory map is captured by a sixth "HOT Spot Analysis" (HSA) track that presents an integrated view of these 41 data sets, identifying regions that may be bound by up to 24 different transcription factors.

Look for these transcription factor binding site (TFBS) data sets with other "Mapped features" in the tracks selection menu at the bottom of the GBrowse page. Click on a binding site to learn more about the bound transcription factors and other experimental details.