Abdu et al., 2006, Development 133(8): 1477--1484

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Abdu et al., 2006, Development 133(8): 1477--1484
FlyBase Identifier FBrf0190267
FlyBase URL http://flybase.org/reports/FBrf0190267.html
Publication Type paper
Publication Year 2006
PubMed ID 16540510
PubMed URL http://www.ncbi.nlm.nih.gov/pubmed/16540510


spn-F encodes a novel protein that affects oocyte patterning and bristle morphology in Drosophila.


The anteroposterior and dorsoventral axes of the Drosophila embryo are established during oogenesis through the activities of Gurken (Grk), a Tgfalpha-like protein, and the Epidermal growth factor receptor (Egfr). spn-F mutant females produce ventralized eggs similar to the phenotype produced by mutations in the grk-Egfr pathway. We found that the ventralization of the eggshell in spn-F mutants is due to defects in the localization and translation of grk mRNA during mid-oogenesis. Analysis of the microtubule network revealed defects in the organization of the microtubules around the oocyte nucleus. In addition, spn-F mutants have defective bristles. We cloned spn-F and found that it encodes a novel coiled-coil protein that localizes to the minus end of microtubules in the oocyte, and this localization requires the microtubule network and a Dynein heavy chain gene. We also show that Spn-F interacts directly with the Dynein light chain Ddlc-1. Our results show that we have identified a novel protein that affects oocyte axis determination and the organization of microtubules during Drosophila oogenesis.

Genes from Reference

Gene(s) Dmel\cnn, Dmel\αTub67C
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