Adachi-Yamada et al., 1999, Mol. Cell. Biol. 19(3): 2322--2329

From FlyBase Wiki
Jump to: navigation, search
Adachi-Yamada et al., 1999, Mol. Cell. Biol. 19(3): 2322--2329
FlyBase Identifier FBrf0106118
FlyBase URL http://flybase.org/reports/FBrf0106118.html
Publication Type paper
Publication Year 1999
PubMed ID 10022918
PubMed URL http://www.ncbi.nlm.nih.gov/pubmed/10022918

Title

p38 mitogen-activated protein kinase can be involved in transforming growth factor beta superfamily signal transduction in Drosophila wing morphogenesis.

Abstract

p38 mitogen-activated protein kinase (p38) has been extensively studied as a stress-responsive kinase, but its role in development remains unknown. The fruit fly, Drosophila melanogaster, has two p38 genes, D-p38a and D-p38b. To elucidate the developmental function of the Drosophila p38's, we used various genetic and pharmacological manipulations to interfere with their functions: expression of a dominant-negative form of D-p38b, expression of antisense D-p38b RNA, reduction of the D-p38 gene dosage, and treatment with the p38 inhibitor SB203580. Expression of a dominant-negative D-p38b in the wing imaginal disc caused a decapentaplegic (dpp)-like phenotype and enhanced the phenotype of a dpp mutant. Dpp is a secretory ligand belonging to the transforming growth factor beta superfamily which triggers various morphogenetic processes through interaction with the receptor Thick veins (Tkv). Inhibition of D-p38b function also caused the suppression of the wing phenotype induced by constitutively active Tkv (TkvCA). Mosaic analysis revealed that D-p38b regulates the Tkv-dependent transcription of the optomotor-blind (omb) gene in non-Dpp-producing cells, indicating that the site of D-p38b action is downstream of Tkv. Furthermore, forced expression of TkvCA induced an increase in the phosphorylated active form(s) of D-p38(s). These results demonstrate that p38, in addition to its role as a transducer of emergency stress signaling, may function to modulate Dpp signaling.

Genes from Reference

Gene(s) Dmel\dpp, Dmel\w
Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox