Adams et al., 2003, Science 299(5604): 265--267

From FlyBase Wiki
Jump to: navigation, search
Adams et al., 2003, Science 299(5604): 265--267
FlyBase Identifier FBrf0155463
FlyBase URL http://flybase.org/reports/FBrf0155463.html
Publication Type paper
Publication Year 2003
PubMed ID 12522255
PubMed URL http://www.ncbi.nlm.nih.gov/pubmed/12522255

Title

Drosophila BLM in double-strand break repair by synthesis-dependent strand annealing.

Abstract

Bloom syndrome, characterized by a predisposition to cancer, is caused by mutation of the RecQ DNA helicase gene BLM. The precise function of BLM remains unclear. Previous research suggested that Drosophila BLM functions in the repair of DNA double-strand breaks. Most double-strand breaks in flies are repaired by homologous recombination through the synthesis-dependent strand-annealing pathway. Here, we demonstrate that Drosophila BLM mutants are severely impaired in their ability to carry out repair DNA synthesis during synthesis-dependent strand annealing. Consequently, repair in the mutants is completed by error-prone pathways that create large deletions. These results suggest a model in which BLM maintains genomic stability by promoting efficient repair DNA synthesis and thereby prevents double-strand break repair by less precise pathways.

Genes from Reference

Gene(s) Dmel\w
Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox