Albalat et al., 1994, Genetica 94(1): 27--36

Albalat et al., 1994, Genetica 94(1): 27--36
FlyBase Identifier	FBrf0079843
FlyBase URL	http://flybase.org/reports/FBrf0079843.html
Publication Type	paper
Publication Year	1994
PubMed ID	7729694
PubMed URL	http://www.ncbi.nlm.nih.gov/pubmed/7729694

Title

Analysis of nucleotide substitutions and amino acid conservation in the Drosophila Adh genomic region.

Abstract

The homologous genomic region that contains two paralogous genes, Adh and Adh-dup, was compared in several Drosophila species. Sequences were analyzed as follows: a) At the nucleotide level, Ka and Ks values were determined for each pair of species. Ka-Adh and Ka-Adh-dup are not significantly different. However, Ks-Adh values are significantly lower than Ks-Adh-dup, which are more variable. In agreement with other reports, lower Ks values for Adh correlate with a high level of gene expression and relatively high percentage of G+C content in the third codon position, while the opposite applies to Adh-dup. b) At the protein level, amino acid comparisons reveal conserved regions shared by ADH and ADH-DUP, which have been assigned to known functional domains. Key residues for dehydrogenasic function are also found in ADH-DUP, thus pointing to a dehydrogenase activity for ADH-DUP, albeit very different from that of ADH.

Genes from Reference

Gene(s)	Dmel\Adh