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Ally et al., 2009, J. Cell Biol. 187(7): 1071--1082

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Ally et al., 2009, J. Cell Biol. 187(7): 1071--1082
FlyBase Identifier FBrf0209566
FlyBase URL http://flybase.org/reports/FBrf0209566.html
Publication Type paper
Publication Year 2009
PubMed ID 20038680
PubMed URL http://www.ncbi.nlm.nih.gov/pubmed/20038680

Title

Opposite-polarity motors activate one another to trigger cargo transport in live cells.

Abstract

Intracellular transport is typically bidirectional, consisting of a series of back and forth movements. Kinesin-1 and cytoplasmic dynein require each other for bidirectional transport of intracellular cargo along microtubules; i.e., inhibition or depletion of kinesin-1 abolishes dynein-driven cargo transport and vice versa. Using Drosophila melanogaster S2 cells, we demonstrate that replacement of endogenous kinesin-1 or dynein with an unrelated, peroxisome-targeted motor of the same directionality activates peroxisome transport in the opposite direction. However, motility-deficient versions of motors, which retain the ability to bind microtubules and hydrolyze adenosine triphosphate, do not activate peroxisome motility. Thus, any pair of opposite-polarity motors, provided they move along microtubules, can activate one another. These results demonstrate that mechanical interactions between opposite-polarity motors are necessary and sufficient for bidirectional organelle transport in live cells.

Genes from Reference

Gene(s) Dmel\Khc
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