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| Term | bacterial display | ID (Ontology) | MI:0009 (Molecular Interactions) |
| Definition | The protein of interest is presented on the outer membrane of Gram negative bacteria by expressing it as a fusion partner to peptide signals that direct heterologous proteins to the cell surface. For instance, a single chain Fv (scFv) antibody fragment, consisting of the variable heavy and variable light domains from two separate anti-digoxin monoclonal antibodies, was displayed on the outer membrane of Escherichia coli by fusing it to an Lpp-OmpA. Similar systems have also been developed for gram positive bacteria. Fluorescence-activated cell sorting (FACS), is used to specifically select clones displaying a protein binding to scFv-producing cells.[ PubMed:10436088 PubMed:8248129 ] | ||
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fluorescence technology |__fluorescence-activated cell sorting__ affinity technology | |__display technology___________________| bacterial display |
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display technology fluorescence-activated cell sorting |
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