FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
CV Term Report
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Term protein complementation assay ID (Ontology) MI:0090 (Molecular Interactions)
Definition In the protein-fragment complementation assay, the proteins of interest (\"Bait\" and \"Prey\") are covalently linked at the genetic level to incomplete fragments of a third protein (known as the \"reporter\") and are expressed in vivo, Interaction between the \"bait\" and the \"prey\" proteins brings the fragments of the \"reporter\" protein in close enough proximity to allow them to reform and become the functional reporter protein. Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.[ PubMed:11495741 ]
Also Known As "complementation" ; "PCA"
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 protein complementation assay (all annotations which use CV term, excluding "NOT" statements)       4
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  interaction detection method
   |__experimental interaction detection
       |__protein complementation assay  5990 rec.
           |__adenylate cyclase complementation
           |__beta galactosidase complementation 1 rec.
           |__beta lactamase complementation
           |__bimolecular fluorescence complementation 599 rec.
           |__dihydrofolate reductase reconstruction 1 rec.
           |__mammalian protein protein interaction trap
           |__protein kinase A complementation
           |__reverse ras recruitment system
           |__split luciferase complementation 59 rec.
           |   |__split firefly luciferase complementation
           |   |__Split renilla luciferase complementation
           |__tox-r dimerization assay 1 rec.
           |__transcriptional complementation assay 5400 rec.
               |__lambda repressor two hybrid
               |__lex-a dimerization assay
               |__membrane yeast two hybrid(+) 4 rec.
               |__one hybrid 9 rec.
               |__reverse two hybrid
               |__three hybrid(+) 54 rec.
               |__two hybrid(+) 5363 rec.
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Is a experimental interaction detection
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Synonyms
  • "complementation" EXACT PSI-MI-short
    "PCA" EXACT PSI-MI-alternate
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