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General Information
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| Term |
mammalian protein protein interaction trap |
ID (Ontology) |
MI:0231 (Molecular Interactions) |
| Definition |
The MAPPIT(mammalian protein-protein interaction Trap) is a screening method for protein-protein interaction in mammalian cells, based on the reconstitution of a membrane STAT (signal transducers and activators of transcription) receptor. The bait protein is fused to a STAT recruitment-deficient receptor and the prey protein to a functional STAT recruitment sites. In such a configuration, a given baitprey interaction restores a STAT-dependent responses leading to the expression of a reporter gene. This system, enable to demonstrate not only protein interaction but also modification-independent and tyrosine phosphorylation- dependent interactions.[ PubMed:12853652 ] |
| Also Known As |
"mappit" |
| Comment |
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Links to External Ontologies
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Records annotated with this term OR any of its CHILD TERMS
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