FB2026_02 , released June 18, 2026

CV Term Report

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General Information

Term

enzymatic footprinting

ID (Ontology)

MI:0605 (Molecular Interactions)

Definition

Binding sites are identified by altered reactivity of a complex to an enzymatic probe compared to the unbound molecules. Residues in close contact with the binding partner are protected from cleavage by the enzyme. When these enzymes are administrated to intact cells, the pattern of protection from the probes identifies the location of DNA-protein or protein-protein interactions in vivo.[ PubMed:8238889 ]

Also Known As

"enzymatic footprint"

Comment

Links to External Ontologies

Annotations

Records annotated with this term OR any of its CHILD TERMS

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Direct annotations: records annotated with this exact term (child terms are NOT included)

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
enzymatic footprinting (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

  biochemical
   |__footprinting
       |__enzymatic footprinting  2 rec.
           |__isotope label footprinting
           |__methylation interference assay
           |__nuclease footprinting 1 rec.
           |   |__DNase I footprinting
           |__protease accessibility laddering
           |__uracil interference assay

Spanning Tree View Settings
Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	footprinting
Part of
Synonyms & Secondary IDs
Synonyms
	"enzymatic footprint" EXACT PSI-MI-short
Secondary IDs

External Crossreferences & Linkouts