Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Term | reverse two hybrid | ID (Ontology) | MI:0726 (Molecular Interactions) |
| Definition | Yeast strains are generated in which expression of DB-X/AD-Y or DBPX hybrid proteins is toxic under particular conditions (negative selection). Under these conditions, dissociation of an interaction should provide a selective advantage thereby facilitating detection: a few growing yeast colonies in which DB-X/AD-Y (or DBPX/binding site) fail to interact should be identified among many nongrowing colonies containing interacting DB-X/AD-Y or DBPX/binding site.[ PubMed:8816797 ] | ||
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protein complementation assay |__transcriptional complementation assay |__reverse two hybrid |
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| Is a | transcriptional complementation assay | ||
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