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General Information
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| Term |
miniaturized immunoprecipitation |
ID (Ontology) |
MI:0946 (Molecular Interactions) |
| Definition |
Combines on-chip in-vitro protein synthesis with an in situ microfluidic affinity assay. Co-spotted DNA microarray containing a linear template encoding the proteins is aligned and bonded with a microfluidic device that creates chambers in the array chip. The experiment consists of three main stages: (i) a biotinylated antibody that recognizes the bait protein on a circular area in deposited inside each individual chamber; (ii) proteins are expressed in vitro by transcription and translation of DNA spotted on the chip; (iii) the bait is immobilized on the chamber surface by the antibody and the chamber is washed, retaining only interacting bait-prey pairs.[ PubMed:19098921 ] |
| Also Known As |
" protein interaction network generator" ; "mIP" ; "ping" |
| Comment |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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No relevant statements available
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Relationships