FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Term split firefly luciferase complementation ID (Ontology) MI:1204 (Molecular Interactions)
Definition Two proteins of interest, a bait and prey, which are genetically fused to amino- and carboxy-terminal fragments of firefly (Photinus pyralis) luciferase, are transiently expressed. Physical interactions of these bait and prey proteins reconstitute some of the luciferase activity and result in light emission in the presence of the luciferase substrate.[ PubMed:20734273 PubMed:22070901 ]
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  protein complementation assay
   |__split luciferase complementation
       |__split firefly luciferase complementation
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