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| Term | proline isomerase assay | ID (Ontology) | MI:1236 (Molecular Interactions) |
| Definition | Measurment of the conversion between cis- and trans- peptide bonds formed by the amine group of a proline. Peptide bonds to proline, and to other N-substituted amino acids (such as sarcosine), are able to populate both the cis and trans isomers the cis and trans isomers of the X-Pro peptide bond (where X represents any amino acid) both experience steric clashes with the neighboring substitution and are nearly equal energetically. Hence, the fraction of X-Pro peptide bonds in the cis isomer under unstrained conditions ranges from 10-40%; the fraction depends slightly on the preceding amino acid, with aromatic residues favoring the cis isomer slightly.[ PubMed:9344417 ] | ||
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enzymatic study |__isomerase assay |__proline isomerase assay |
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| Is a | isomerase assay | ||
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