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| Term | validated two hybrid | ID (Ontology) | MI:1356 (Molecular Interactions) |
| Definition | The protein pairs, often initially identified by a separate 2-hybrid screening methodology, are subjected to a rigorous re-analysis which may include independent re-screening of the entire search space, retesting the assay in different strain backgrounds, and multiple retesting of identified protein pairs reversing bait-prey orientations. Orthogonal data from other experimental and bioinformatic approaches may also be used to support the identification of the final high-confidence protein pairs but the primary means of selection must be experimentally based. This method would be expected to identify protein pairs with a higher degree of confidence than any single protein complementation technique.[ PubMed:25416956 ] | ||
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transcriptional complementation assay |__two hybrid |__validated two hybrid |
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| Is a | two hybrid | ||
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