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| Term | par-clip | ID (Ontology) | MI:2188 (Molecular Interactions) |
| Definition | Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) is a biochemical method used for identifying the binding sites of cellular RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs). The method relies on the incorporation of photoreactive ribonucleoside analogs, such as 4-thiouridine (4-SU) and 6-thioguanosine (6-SG) into nascent RNA transcripts by living cells. Irradiation of the cells by UV light of 365 nm induces efficient crosslinking of photoreactive nucleoside-labeled cellular RNAs to interacting RBPs. Immunoprecipitation of the RBP of interest is followed by isolation of the crosslinked and coimmunoprecipitated RNA. The isolated RNA is converted into a cDNA library and deep sequenced using next-generation sequencing technology.\nhttp://en.wikipedia.org/wiki/PAR-CLIP[ PubMed:20371350 PubMed:20644507 ] | ||
| Also Known As | "PAR-CLIP" ; "par-clip" ; "Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation" | ||
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nucleic acid uv cross-linking assay |__clip |__par-clip |
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