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General Information
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| Term |
molecular sieving |
ID (Ontology) |
MI:0071 (Molecular Interactions) |
| Definition |
In sizing columns (gel filtration), the elution position of a protein or of a complex depends on its Stokes radius. Molecules with a radius that is smaller than the bead size are retained and retarded by the interaction with the matrix. The observation that two proteins, loaded on a sieving column, elute in a fraction(s) corresponding to a MW that is larger than the MW of either protein may be taken as an indication that the two proteins interact. Furthermore this technique provides a conceptually simple method for evaluating the affinity of the interaction.[ PubMed:7708014 ] |
| Also Known As |
"Gel Filtration" ; "Size Exclusion Chromatography" ; "Sizing column" |
| Comment |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Interaction Groups |
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molecular sieving (all annotations which use CV term, excluding "NOT" statements) | 528 |
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Relationships