FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
CV Term Report
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General Information
Term protein three hybrid ID (Ontology) MI:0437 (Molecular Interactions)
Definition Two hybrid assay performed with a third protein component co-transfected into a recombinant yeast strain together with a bait and a prey construct. Negative control shows that the interaction between the bait and the prey do not occur when the third protein is not co-transfected.[ PubMed:12052864 PubMed:12761205 PubMed:12935900 ]
Also Known As "bridge assay" ; "protein 3-hybrid" ; "protein tri hybrid" (for all, see Synonyms field below)
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 protein three hybrid (all annotations which use CV term, excluding "NOT" statements)      17
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transcriptional complementation assay
 |__two hybrid____
 |__three hybrid__|
                  protein three hybrid  17 rec.
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Synonyms
  • "bridge assay" EXACT PSI-MI-alternate
    "protein 3-hybrid" EXACT PSI-MI-alternate
    "protein tri hybrid" EXACT PSI-MI-alternate
    "trihybrid" EXACT PSI-MI-alternate
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