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| Term | protein in situ array | ID (Ontology) | MI:0092 (Molecular Interactions) |
| Definition | Protein In Situ Array is a method by which protein arrays are rapidly generated in one step directly from DNA, by cell-free protein expression and simultaneous in situ immobilisation at a surface. Individual genes or fragments are produce by PCR or RT-PCR depending on the source of genetic material using properly designed primers. The PISA is generated by cell-free protein synthesis using coupled transcription and translation to produce a tagged protein, the reaction being carried out on a surface to which the protein adheres as soon as it is synthesised.[ PubMed:11470888 ] | ||
| Also Known As | "PISA" ; "pisa" | ||
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array technology |__protein array |__protein in situ array |__miniaturized immunoprecipitation |
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| Is a | protein array | ||
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