FB2026_01 , released March 12, 2026

CV ID	CV term	Synonyms	Definition
MI:0071	molecular sieving	Gel Filtration [EXACT] Size Exclusion Chromatography [EXACT] Sizing column [EXACT]	In sizing columns (gel filtration), the elution position of a protein or of a complex depends on its Stokes radius. Molecules with a radius that is smaller than the bead size are retained and retarded by the interaction with the matrix. The observation that two proteins, loaded on a sieving column, elute in a fraction(s) corresponding to a MW that is larger than the MW of either protein may be taken as an indication that the two proteins interact. Furthermore this technique provides a conceptually simple method for evaluating the affinity of the interaction.

CV ID

CV term

Synonyms

Definition

MI:0071

Gel Filtration [EXACT]
Size Exclusion Chromatography [EXACT]
Sizing column [EXACT]

In sizing columns (gel filtration), the elution position of a protein or of a complex depends on its Stokes radius. Molecules with a radius that is smaller than the bead size are retained and retarded by the interaction with the matrix. The observation that two proteins, loaded on a sieving column, elute in a fraction(s) corresponding to a MW that is larger than the MW of either protein may be taken as an indication that the two proteins interact. Furthermore this technique provides a conceptually simple method for evaluating the affinity of the interaction.