Dl-enhancer trap construct. - Haenlin, M. (1998)
Subject: FlyBase query Dear Dr. Haenlin, I am curating a paper for FlyBase (Baker and Yu, 1998, Mech. Dev. 74(1,2): 3--14) in which a Dl-enhancer trap construct isolated by you and J.A. Campos-Ortega was used. FlyBase has a record of a number of Dl-enhancer trap constructs but I don't think that that these correspond to your construct. I wrote to the corresponding author on the paper (N. Baker) and he suggested I write to you about the details of the construct. I would be grateful if you could tell me a few details of the construct so that I can confirm whether this construct is new to FlyBase. If it is new to FlyBase I would like to include the details in FlyBase as a personal communication from you, if possible. 1. is there a reference where this construct has been published. 2. is there a designation/line number for the construct e.g. A101, 64B 3. which enhancer trap vector, e.g. PPZ, PlacW is used I look forward to hearing from you, Gillian Millburn \-------------------------------------------------------------- Gillian Millburn. FlyBase (Cambridge), \-------------------------------------------------------------- > >Dear Dr. Haenlin, > >I am curating a paper for FlyBase (Baker and Yu, 1998, Mech. Dev. 74(1,2): >3--14) in which a Dl-enhancer trap construct isolated by you and J.A. >Campos-Ortega was used. > >FlyBase has a record of a number of Dl-enhancer trap constructs but I don't >think that that these correspond to your construct. I wrote to the >corresponding author on the paper (N. Baker) and he suggested I write to >you about the details of the construct. > >I would be grateful if you could tell me a few details of the construct so >that I can confirm whether this construct is new to FlyBase. If it is new >to FlyBase I would like to include the details in FlyBase as a personal >communication from you, if possible. > >1. is there a reference where this construct has been published. >2. is there a designation/line number for the construct e.g. A101, 64B >3. which enhancer trap vector, e.g. PPZ, PlacW is used > Dear Gillian I sendet two lines; both of them are insertion of PlacW in the promotor region of Delta at about the same site (less as 1 kb). For the 8c3 (originating from the Jan's Lab as I know) the LacZ transcription orientation is the same as Delta (the Dl revF10 is a revertant of this enhancer trap line), and for the 1282 (from Seugnet and Haenlin) is in the opposite direction. It exist as I know no references for these lines. If you have further questions, no problem regards Haenlin Marc Centre de Biologie du Developpement Batiment 4R3 118, route de Narbonne 31062 Toulouse > Subject: Re: FlyBase query Dear Marc, thankyou for your reply, it was very helpful. I think I have found the 8c3 line in FlyBase - we have a PlacW insertion in Dl called j8C3 which comes from the Berkeley Genome Project - and I think the j means that it came from the Jan's lab. the 1282 line is new. I would like to include the details from your e-mail about these lines in FlyBase as a personal communication to FlyBase from you. Would that be OK ? Gillian > Subject: Re: FlyBase query >Dear Marc, > >thankyou for your reply, it was very helpful. > >I think I have found the 8c3 line in FlyBase - we have a PlacW insertion >in Dl called j8C3 which comes from the Berkeley Genome Project - and I >think the j means that it came from the Jan's lab. > >the 1282 line is new. > >I would like to include the details from your e-mail about these lines in >FlyBase as a personal communication to FlyBase from you. > >Would that be OK ? > Dear Gillian no problem