FlyBase error report for CG9900 on Thu Apr 13 06:28:37 2000. - Whitfield, E. (2000)
Gene or accession: CG9900 Gene annotation error Genes CG990, FBgn0029627 and mit(1)15, FBgn0004643 should be merged. Comments: Sequence alignment of: 1) AE003424; AAF45794.1 (Celera full length 721aa sequence, strain y; cn bw sp) 2) X64390; CAB76122.1 (Williams et al, Medline: 92363920, full length 721aa sequence, strain unidentified) 3) AL138972; CAB72295.1 (EDGP full length 721aa sequence, strain Oregon-R) 4) AL121804; CAB65854.1 (EDGP C-terminal 461aa fragment, strain Oregon-R) demonstrates that CG9900 and mit(1)15 are in fact the same gene, clustal shown below. I was concerned that the map position of CG9900 is given as 3A4 and mit(1)15 as 3A9, but looking at the evidence for mit(1)15: from star coded flat file of genes \*c 3A9 \*c Left limit from inclusion within Df(1)z-deb81 (FBrf0049883) \*c Right limit from inclusion within Df(1)z-EM46 (FBrf0054120) and at the individual Df breakpoint positions, mit(1)15 is actually between 3A1 (Df(1)z-deb81) and 3A9 (Df(1)z-EM46), making 3A4 a possible position. I hope with this evidence you agree with me, Regards Eleanor Whitfield Swiss-Prot annotator CLUSTAL W (1.8) multiple sequence alignment Celera sequence is represented by Swiss-prot accession number: Q9W4X9 CAB72295 MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFLPN CAB65854 \------------------------------------------------------------ CAB76122 MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFMPN Q9W4X9 MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFLPN CAB72295 NTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHV CAB65854 \------------------------------------------------------------ CAB76122 NTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHV Q9W4X9 NTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHV CAB72295 LRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECY CAB65854 \------------------------------------------------------------ CAB76122 LRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECY Q9W4X9 LRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECY CAB72295 ETIKVKYHVQAYMLQQSLQERFDRLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQE CAB65854 \------------------------------------------------------------ CAB76122 ETIKVKYHVQAYMLQQSLQERFARLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQE Q9W4X9 ETIKVKYHVQAYMLQQSLQERFDRLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQE CAB72295 PYNPARLAEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQ CAB65854 \--------------------MRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQ CAB76122 PYNPARLCEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQ Q9W4X9 PYNPARLCEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAQLRPNYKQ CAB72295 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS CAB65854 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS CAB76122 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS Q9W4X9 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS CAB72295 TLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD CAB65854 TLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD CAB76122 TLCEDAAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD Q9W4X9 TLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD CAB72295 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS CAB65854 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS CAB76122 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS Q9W4X9 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS CAB72295 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT CAB65854 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT CAB76122 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT Q9W4X9 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT CAB72295 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET CAB65854 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET CAB76122 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET Q9W4X9 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET CAB72295 VYNATFCELINTFVAELIRRVFTLRHISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL CAB65854 VYNATFCELINTFVAELIRRVFTLRHISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL CAB76122 VYNATFCELINTFVAELIRRVFTLRDISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL Q9W4X9 VYNATFCELINTFVAELIRRVFTLRDISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL CAB72295 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI CAB65854 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI CAB76122 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI Q9W4X9 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI CAB72295 V CAB65854 V CAB76122 V Q9W4X9 V \* Differences between y; cn bw sp and Oregon-R (AL138972; CAB72295 and AL121804; CAB65854) 248 248 C \-> A 293 294 QL \-> HV 626 626 D \-> H Differences between y; cn bw sp and Williams et al (X64390; CAB76122) 58 58 L \-> M 203 203 D \-> A 293 294 QL \-> HV 366 366 V \-> A