Pi3K92E constructs and insertions. - Parks, A. (2004)
Subject: Pi3K92E constructs and insertions The following information was provided by Annette Parks of Exelixis, Inc. In PUAS-Pi3K92E.Exel, the coding region of wild type Pi3K92E (FBgn0015279) was cloned into PExpress-UAS. P{UAS-Pi3K92E.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{UAS-Pi3K92E.Exel}3 is a homozygous viable and fertile, third chromosome insertion. In PGMR-Pi3K92E.Exel, the coding region of wild type Pi3K92E was cloned into PExpress-GMR. P{GMR-Pi3K92E.Exel}2 is a homozygous viable and fertile, second chromosome insertion. In PUAS-Pi3K92E.A2860C, Pi3K92E sequences with a A2860C nucleotide change were cloned into PExpress-UAS. The A2860C mutation changes an Asp to Ala in the ATP binding site and results in a dominant negative allele. P{UAS-Pi3K92E.A2860C}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{UAS-Pi3K92E.A2860C}3 is a homozygous viable and fertile, third chromosome insertion. In PGMR-Pi3K92E.A2860C, Pi3K92E sequences with a A2860C nucleotide change were cloned into PExpress-glass. The A2860 mutation changes an Asp to Ala in the ATP binding site and results in a dominant negative allele. P{GMR-Pi3K92E.A2860C}2 is a homozygous viable and fertile, second chromosome insertion. P{GMR-Pi3K92E.A2860C}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. In PGMR-Pi3K92E.CAAX, Pi3K92E coding sequence with a farnesylation signal (CAAX) added to the 3' end were cloned into PExpress-glass. The encoded protein is constitutively active. P{GMR-Pi3K92E.CAAX}2 is a second chromosome insertion. In PUAS-Pi3K92E.CAAX, Pi3K92E coding sequence with a farnesylation signal (CAAX) added to the 3' end were cloned into PExpress-UAS. The encoded protein is constitutively active. P{UAS-Pi3K92E.CAAX}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion.