wfs1 constructs and insertions. - Parks, A. (2004)
Subject: wfs1 constructs and insertions The following information was provided by Annette Parks of Exelixis, Inc. CG4917 (FBgn0039003) is the fly homolog of the human Wolfram syndrome 1 (WFS1) gene. The human protein is also called wolframin in some references. In PGMR-wfs1.Exel, full length wfs1 was cloned into PExpress-glass. The sequence of wfs1 in this construct and the two others below is not identical to other sequenced clones. Its sequence is: MATWTQNEPTGVTKRRRWNLEDRASLNKLKHHIAEEGCPQMQYDLAKELLDNSIEPNLAKGNQSQKAVNWLVSAAHNGH EDAVKLLRQCYNDGSGITAENTDEVRRCLAMTPGERAARKAARELFACLSNGNEHITPKQLERKMRRIYNLQRKRRRRD DDRSSSSSEGEQEPECEPLEDVPTIDLANVERRRLITEAHLVSAASNYSAGQMPSVNDALTLSVPDPRSLDHVPCFYRM IFHPLIFFTLFYHRLLNLIVSIPNVIPLSVRCSVLVAISWWSSRHMLPLVSYYLSLGIMIWATCKMLKTKQQFVDFRIW SGLFLSYGDQNIEADIAEHRFLRNNMKPYLYFFCAFICNLIVYPLVTDAWLPHSELTIISGALTFITMCVSMYASSHQL PDWLVIVSFAVNVLAKYPYEMDEVVSTRWRFLDLRVPTFSSFVIGNGIEFCLNCRTALYLFIPVLLIMMAKRSRWHGVY TFLIPHCVTLSWLQVCIATSQSSTVFGVMRAALGLAGIVLFLPLFGIVALLVPVFVAIDSLGLASEQLRWGSTALACGL VVVLSCILALNRATQKYITMLQLITAITTACLLVLPYMTSSFKDTPRFNAMPRAGLHSLSETNTLPWDRFHALCAQPVH EQPNKIKAQLRCSLLNGMPVIWEGSVTKVEISRVSNFLEDTIANYLPVWLGRMLRCLHGENISQHFKCDPKLDAQCEEW RSVFKTFNAQSGSCTLQRWNRYEYELLVKVGTKRSGRLLGRSTTTDVILRAHHDFGNFTRLLSEGDVVLFYGILHNSRL LADNVQVKLKTIECVECRSRDLGTASIERVVAASPMDARLQDLMRGIKYLLNALLNPLITFK P{GMR-wfs1.Exel}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{GMR-wfs1.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{GMR-wfs1.Exel}3 is a homozygous viable and fertile, third chromosome insertion. In PUAS-wfs1.Exel, full length wfs1 was cloned into PExpress-UAS. P{UAS-wfs1.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{UAS-wfs1.Exel}3 is a homozygous viable and fertile, third chromosome insertion. In Psev-wfs1.Exel, full length wfs1 was cloned into either PExpress-sev1x or PExpress-sev3x. P{sev-wfs1.Exel}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{sev-wfs1.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{sev-wfs1.Exel}3 is a homozygous viable and fertile, third chromosome insertion.