unc-13[P84200]. - Cook, K. (2005)
Subject: unc-13[P84200] Hi folks-- We need to sort out some confusion associated with unc-13[P84200]. unc-13[P84200] was described in Aravamudan et al. (FBrf0111780) as a P insertion in the unc-13 gene. They stated that the mutation was obtained from the Umea Stock Centre as their stock P84200. The genotype of stock P84200 was ry[506]; P{ry[+t7.2]=ry11}l(4)16[1]/ci[D] when it was first placed in the Umea collection. The name of this insertion was later changed to Pry11l(4)ry16[1], though this change was apparently never made in the Umea stock list. The FlyBase insertion entry for Pry11l(4)ry16[1] is currently FBti0000917 and the allele entry is FBal0032045. Aravamudan et al. showed that excision of the insertion reverted the lethality, so there should be no problem in merging the allele entries for unc-13[P84200] and l(4)ry16[1] and the gene entries for unc-13 and l(4)ry16. There are two additional confusing points. First, Kueppers et al. (FBrf0159054) used unc-13[P84200] and cited Aravamudan et al., but mistakenly called the allele unc-13[P8420] in their Table 1. Consequently, unc-13[P8420] (currently FBal0143143) should be considered a synonym for unc-13[P84200]. Second, there are two loci which have been called l(4)16. One is l(4)ry16 (=unc-13). The other is l(4)102EFb (FBgn0004537). l(4)102EFb[1] (FBal0011492) was a spontaneous mutation isolated from a natural population by Hochman. At some point, the genotype of Umea stock P84200 was mistakenly changed from ry[506]; P{ry[+t7.2]=ry11}l(4)16[1]/ci[D] to ry[506] ; P{ry[+t7.2]=ry11}l(4)102EFb[1]/ci[D] based on false synonymy. l(4)102EFb and unc-13 are different loci, though anyone who used Umea stock P84200 might think otherwise. Thanks! Kevin