Isolation and characterization of Df(2L)BSC144. - Christensen, S.; Cook, K. (2006)
Isolation and characterization of Df(2L)BSC144 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC144 was isolated as a FLP recombinase-induced recombination event involving PBacRBe01839 and PXPd08708. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBacRBe01839/PXPd08708 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.RB3BSC144 from the segment of PBacRBe01839 to the left of its FRT site and the segment of PXPd08708 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. Exelixis, Inc. determined the insertion site of PXPd08708 to be at Release 3 genomic coordinate 10450004 on chromosome arm 2L. The cytological breakpoints of Df(2L)BSC144 predicted from the transposable element insertions sites using release 3 coordinates are 31B1;31E5. It failed to complement chico[1], trk[1] and bsk[1].